E-ISSN 2564-615X
Research Article
Structures of cell-wall glycopolymers of Lactococcus lactis BIM B-1024
1 N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia  
2 N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia; Higher Chemical College of the Russian Academy of Sciences, D. I. Mendeleev University of Chemical Technology of Russia, Moscow, Russia  
3 Institute of Bioorganic chemistry, National Academy of Sciences of Belarus, Minsk, Belarus  
4 Institute of Microbiology, National Academy of Sciences of Belarus, Minsk, Belarus  
Eurobiotech J 2017; 1: 41-45
DOI: 10.24190/ISSN2564-615X/2017/01.07
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Abstract

Glycopolymers of two types were isolated from the cell wall of Lactococcus lactis BIM B-1024 by stepwise extraction with cold and hot 10% CCl3 CO2 H and separated by anion-exchange gel chromatography. The following structures of the glycopolymers were established by sugar analysis, dephosphorylation with 48% HF, 1D and 2D NMR spectroscopy, and ESI-MS:

 

 

Polysaccharides with the same or similar structures to PSI have been found earlier in various Lactobacillus species, whereas, to our knowledge, the structure of PSII is new.

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