Culture cells in a model of microgravity
Institute of Physiology, National Academy of Sciences of Belarus, Minsk, Belarus
Eurobiotech J 2017; 1: 93-94
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The main objective of the work was to clarify the question – how will cell cultures functional state change after microgravity simulation when the shift in full strength direction takes place? Proliferation processes and apoptosis intensity in cell lines of rat glioma and human fibroblasts were compared in changing the position of flasks with cell culture in relation to the horizon. The detection of apoptosis and necrosis processes was carried out using flow cytofluorimetry. It was found that the change in full strength direction provides an inhibitory effect on tumor glial cells and fibroblasts’ proliferative activity enhances along with inhibition of apoptotic processes. Intensification of apoptotic processes in glioma cells and attenuation of cell death processes in normal cells – fibroblasts – are the result of cell cooperation disturbance.